The Torres del Paine National Park and environs has four types of vegetation - pre-Andean scrubland on the banks of rivers and lakes; Magallanes forest made up of species of the Nothofagus genus; Magallanes tundra, typified by the presence of bent-over bushes, cushions of plants and pasturelands; and high altitude vegetation above the forests, which disappears as the altitude increases.

This diversity of environments leads to presence of a large number of mammals, including especially the Puma (Felis concolor), Guemal (Hippocamelus bisulcus), Guanaco (Lama guanicoe), Chilla Fox (Canis griseus), Culpeo Fox (Canis culpaeus) and Skunk (Conepactus chinga).

The park has a wide variety of bird fauna, with about 100 visible species, including especially Andean Condor (Vultur gryphus), Black-chested Buzzard Eagle (Geranoaetus melanoleucus), Black-necked Swan (Cygnus melanocoryphus), Chilean Flamingo (Phoenicopterus chilensis), Spectacled Duck (Anas specularis), Magellanic Woodpecker (Campephilus magellanicus), Ringed Kingfisher (Ceryle torquata), Patagonian Sierra-Finch (Phrygilus patagonicus), Common Diuca-Finch (Diuca diuca) and Rufous-collared Sparrow (Zonotrichia capensis).